【 】OEM:MMlV,H,minus,RTase

Reliable,quality,and,low,price.,Low,to,10$/10000,Units(,50,applications)

M-MLV,Reverse,Transcriptase,,RNase,H,Minus,,Point,Mutant:
Description:,Moloney,Murine,Leukemia,Virus,Reverse,Transcriptase,,RNase,H,Minus,,Point,Mutant,(M-MLV,RT,(H–)),is,an,RNA-dependent,DNA,polymerase,that,can,be,used,in,cDNA,synthesis,with,long,RNA,templates,(>5kb).,The,lack,of,RNase,H,activity,is,germane,to,this,application,in,that,this,activity,can,start,to,degrade,template,when,incubation,times,are,long,,as,they,may,need,to,be,when,mak,ing,long,cDNAs.,Although,many,researchers,are,successful,in,using,M-MLV,RT,(H,),for,analytical,and,some,preparative,cDNA,applications,,reverse,transcriptases,lacking,RNase,H,activity,provide,another,option,for,the,preparation,of,long,cDNAs,and,libraries,containing,a,high,percentage,of,full-length,cDNA.
An,application,of,M-MLV,RT,(H–),is,first-strand,synthesis,of,cDNA,from,RNA,molecules.


Enzyme,Storage,Buffer:,M-MLV,Reverse,Transcriptase,,RNase,H,Minus,,Point,Mutant,is,supplied,in,20mM,Tris-HCl,(pH, ),,200mM,NaCl,, ,EDTA,,1mM,DTT,, %,Nonidet?,P-40,and,50%,glycerol.
M-MLV,Reverse,Transcriptase,5X,Reaction,Buffer,:,When,the,M-MLV,Reverse,Transcriptase,5X,Reaction
Buffer,supplied,with,this,enzyme,is,diluted,1:5,,it,has,a,composition,of,50mM,Tris-HCl,(pH, ),,75mM,KCl,,3mM,MgCl2,and,10mM,DTT.
Source:,Purified,from,an,E.,coli,strain,expressing,a,recombinant,enzyme,
Storage,Temperature:,Store,at,–20°C.,,of,the