Catalog Number: 14120-1, 14120-2 Amount: 50μg/50μl, 100μg/100μl Swiss-Prot No. :P31749 Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. Storage/Stability: Store at -20℃/1 year Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human AKT around the phosphorylation site of Sersine 473 Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Specificity/Sensitivity:Phospho-AKT (Ser473) Antibody detects endogenous levels of AKT only when phosphorylated at Sersine 473 Reactivity: Human,Mouse,Rat Applications: Predicted MW: 60kd WB:1:500~1:2000 IHC:1:50-200 IF/ICC:1:100-500 Western blot analysis on 293 cell lysate using Phospho-Akt(Ser473) Antibody Background :an AGC kinase that plays a critical role in controlling the balance between survival and AP0ptosis. Phosphorylated and activated by PDK1 in the PI3 kinase pathway. Mediates survival signals downstream of PI3 kinase and several growth factor receptors by phosphorylating AP0pototic proteins. First found in a mouse transforming retrovirus. Tumorigenic in a mouse lymphoma model and activated (by phospho-Akt staining) and/or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Inhibitor: RX-0201. Substrates include tuberin, Bad, Forkhead transcription factors, caspase-9, and glycogen synthase kinase-3.