Catalog Number: T503-1 T503-1 Amount: 50μg/50μl 100μg/100μl Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. Storage/Stability: Store at -20℃/1 year Immunogen: The antiserum was produced by immunizing rabbit with a synthesized DYKDDDDK peptide (FLAG® epitope), FLAG® is a registered trademark of Sigma-Aldrich Co.. Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Specificity/Sensitivity:Anti-DYKDDDDK Antibody detects exogenously expressed DYKDDDDK proteins in cells.The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® M2 antibodies) fused to either the amino- or carboxy- terminus of targeted proteins. Reactivity: All Background : Epitope tags are artificial epitopes useful for the labeling and detection of proteins. Epitope tags - short amino acid sequences are 'fused' to the N- or C-terminus of the protein. References: Terpe K. (2003) Appl Microbiol Biotechnol; 60(5):523-533. Einhauer A, et al. (2001) J Biochem Biophys Methods; 49(1-3):455-465. Brizzard, B.L. et al. (1994) Biotechniques; 16:730–735.