Catalog Number: 11003-1, 11003-2 Amount: 50μg/50μl, 100μg/100μl Swiss-Prot No. :P05412 Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. Storage/Stability: Store at -20℃/1 year Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human c-Jun around the phosphorylation site of serine 73 (L-A-SP-P-E). Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. Specificity/Sensitivity:c-Jun (phospho-Ser73) antibody detects endogenous levels of c-Jun only whenphosphorylated at serine 73 Reactivity: Human,Mouse,Rat Applications: Predicted MW: 43kd WB: 1:500~1:1000 IHC: 1:50~1:100 Western blot analysis of extracts from PC14 cells using c- Jun (phospho-Ser73) antibody (#11003). Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (phospho- Ser73) antibody (#11003). Background : Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. References： Sevilla A, et al. (2004) Oncogene.23 (55): 8950-8958. Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135. Binetruy B, et al. (1991) Nature. 351:122-127. Smeal T, et al. (1991) Nature. 354: 494-496. Derijard B, et al. (1994) Cell. 76:1025-1037.