Catalog Number: 11004-1, 11004-2 Amount: 50μg/50μl, 100μg/100μl Swiss-Prot No. :P19419 Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. Storage/Stability: Store at -20℃/1 year Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Elk-1around the phosphorylation site of serine 383 (T-L-SP-P-I). Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site Specificity/Sensitivity: Elk-1 (phospho-Ser383) antibody detects endogenous levels of Elk-1 only when phosphorylated at serine 383. Reactivity: Human,Mouse,Rat Applications: Predicted MW: 62 kd WB: 1:500~1:1000 IHC:1:50~1:100 UV - + Western blot analysis of extract from HeLa cells undealt or treated with UV using Elk-1 (phospho-Ser383) antibody (#11004). Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (Phospho-Ser383) antibody (#11004). Background : Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein were found for this gene. References: Xing J, et al. (1996) Science. 273(5277): 959-963. Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104. Marais R, et al. (1993) Cell 73: 381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824.