Catalog Number: 53339 Amount: 100μg/100μl Swiss-Prot No. :P12004 Gene name:pcna Gene id:5111 Clone Number：2E1-G10-H10 Form of Antibody:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol Storage/Stability: Store at -20℃/1 year Immunogen: Purified recombinant human PCNA protein fragments expressed in E.coli Purification: affinity-chromatography Specificity/Sensitivity:This antibody detects endogenous levels of PCNA and does not corss-react with related proteins Reactivity: Human,Mouse,Rat,Monkey,Hamster Applications: Predicted MW: 36kd WB: 1:1000 ICC/IHC:1:100 Western blot detection of PCNA in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000diluted).Predicted band size:36KDa.Observed band size:36KDa. Background:. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.