Catalog Number: 53618 Amount: 100μg/100μl Swiss-Prot No. :P02545 Gene name:lmna Gene id:4000 Clone Number： 3D6-F4-B7 Form of Antibody:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol Storage/Stability: Store at -20℃/1 year Immunogen: Purified recombinant human LaminA protein fragments expressed in E.coli Purification: affinity-chromatography Specificity/Sensitivity:This antibody detects endogenous levels of LaminA and does not corss-react with related proteins Reactivity: Human,Mouse,Rat Applications: Predicted MW: 74kd WB: 1:1000 Western blot detection of Lamin A in K562,C6,3T3 and Hela cell lysates using Lamin A mouse mAb (1:1000diluted).Predicted band size:74KDa.Observed band size:74KDa. Background:. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane.The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B.Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases:Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.