Catalog Number: 11008-1, 11008-2 Amount: 50μg/50μl, 100μg/100μl Swiss-Prot No. :P36507 Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. Storage/Stability: Store at -20℃/1 year Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T). Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. Specificity/Sensitivity:MEK2 (phospho-Thr394) antibody detects endogenous levels of MEK2 only when phosphorylated at threonine 394. Reactivity: Human,Mouse,Rat Applications: Predicted MW: 44kd WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:00~1:200 Western blot analysis of extracts from ovary cancer cells using MEK2 (phospho-Thr394) antibody (#11008). Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK2 (phospho-Thr394) antibody (#11008).Immunofluorescence staining of methanol-fixed HeLa cells using MEK2 (phospho-Thr394) antibody (#11008). Background : Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases References： Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221.